Retrospective overview of a COVID-19 outbreak in Mauritania.

A coronavirus disease 2019 (COVID-19) outbreak is currently ongoing in Mauritania. Until 1 July 2020, Mauritania health authorities reported 41 862 serologic and real-time reverse transcriptase PCR tests performed, of which 4472 (10.7%) were positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Male subjects were significantly more affected (57.1%) than female subjects (42.9%). Individuals of the age groups 15-34 years (35.8%) and 35-54 years (36.6%) were the most affected. There were 129 deaths (2.9%), 1677 recoveries (37.5%) and 2666 active cases (59.6%) of which 2261 (84.8%) were asymptomatic, 394 (14.7%) had mild symptoms and 11 (0.4%) had severe symptoms. A large proportion of fatalities (72%, 85/118) occurred among adults aged ≥55 years. Of 4472 positive cases, 4241 (94.8%) were infected through contact with a confirmed COVID-19 case, 133 (3.0%) had no contact with a confirmed COVID-19 case and 98 (2.2%) were imported. As a response to COVID-19, the Mauritanian authorities announced a set of preventive measures, including closure of land and air borders, night curfew, closure of markets, schools and universities and restriction of movement between cities. Control measures included the systematic testing of symptomatic patients, isolation and management of active cases, contact tracing and quarantine of people who have been in contact with a COVID-19-positive individual. We discuss the efforts of the Mauritanian government to combat this potentially life-threatening pneumonia.

Traumatic renal injury: Five-year experience at a major trauma centre in South Africa.

BACKGROUND: This study is intended to assess the current optimal management of traumatic renal injuries (TRIs), with a focus on high-grade and penetrating injuries. METHODS: The Pietermaritzburg Metropolitan Trauma Service registry was interrogated retrospectively for patients managed for TRI between 1 January 2012 and 31 December 2016. RESULTS: Of 13,315 inured patients treated by the PMTS, 223 (1.7%) had TRIs with an incidence of 1.5 per 100,000 population per year. The majority were males between 20 and 39 years of age. The distribution of mechanism of injury was 56.1% (n = 125) blunt and 43.9% (n = 98) penetrating trauma with no association between mechanism and grade of injury. Penetrating trauma was associated with hollow viscus and diaphragm injuries and blunt trauma with solid organ injuries. A total of 118 patients (52.9%) were managed non-operatively, 60 (26.9%) were not explored at operation, 27 (12.1%) underwent initial nephrectomy and 8 (3.6%) underwent renorraphy. Low-grade injuries (AAST I and II) and high-grade injuries (AAST III-V) were managed without renal intervention (non-operatively or not explored at laparotomy for associated injuries) in 88.7% (n = 87) and 72.0% (n = 91) of cases respectively. Blunt and penetrating injuries were managed without renal intervention in 87.9% (n = 109) and 70% (n = 69) of cases respectively. The initial nephrectomy rate was 1% (n = 1) and 20.6% (n = 26) for low- and high-grade injuries respectively, and 6.5% (n = 8) and 19% (n = 19) for blunt and penetrating injuries respectively. High grade (AAST III-V) injury (OR 14.94; 95% CI 3.36 - 66.34; p<0.001), penetrating mechanism (OR 4.99; 95% CI 1.98 - 12.52; p = 0.001) and metabolic acidosis (OR 2.73; 95% CI 1.04 - 7.20; p = 0.042) were significant risk factors for nephrectomy. Four patients (1.8%) underwent ureteral stent insertion and 2 (0.9%) underwent embolisation. The failure rate of initial non-operative management was 1.1%. The mortality rate was 8.1% (n = 18), but no patients with solitary renal injuries died. CONCLUSION: Even in high-grade injuries and penetrating trauma, the majority of patients with TRI can be managed non-operatively or with the assistance of endourological or endovascular techniques, with good outcomes. Risk factors for nephrectomy include the presence of high-grade injuries, penetrating trauma and metabolic acidosis on presentation.

Dissolution enhancement of gliclazide using ultrasound waves and stabilizers in liquid anti-solvent precipitation.

The absorption rate of gliclazide is slow and variable among subjects probably due to poor dissolution from the dosage form. The objective of this study was to enhance the dissolution rate of gliclazide by reducing the particle size. Gliclazide was precipitated from an acetone solution by adding an antisolvent (water) containing stabilizers. A combination of jets (flow rate of 20 ml/min), ultrasound, HPMC 4000, and sodium dodecyl sulfate was used to control particle size and particle size distribution. The effects of concentration of stabilizers, initial drug concentration in solution, time of insonation, antisolvent-to-solvent ratio, and ultrasound power on particle size and particle size distribution were studied. Precipitated drug particles were characterized by laser diffraction particle size analysis, SEM, FTIR spectroscopy, DSC, powder x-ray diffraction and in-vitro dissolution. With increasing almost all the studied parameters, the particle size of gliclazide initially decreased, exhibited a minimum, and then increased. Drug particles of glicazide with a mean particle size of 1.56 ± 0.09 µm and a narrow size distribution (d10/d50/d90 = 0.67/1.67/2.26) were precipitated as compared to unprocessed gliclazide with a mean particle size of 10.67 ± 0.04 µm and a wide size distribution (d10/ds50/d90 = 4.53/9.88/18.03). SEM images indicated changes in the particle morphology. Powder x-ray diffraction patterns and DSC curves indicated no changes in the chemical properties but only decrease in crystallinity and/or particle size. The dissolution rate was enhanced 2.55-fold. In conclusion, drug particles with small size and narrow size distribution were precipitated by selecting favorable process conditions, and dissolution was enhanced several folds.

A common mutation in the CBS gene explains a high incidence of homocystinuria in the Qatari population.

We report the results of a study carried out to delineate genetic and epidemiological aspects of homocystinuria in the Qatari population. Sixty-four patients with homocystinuria (37 males, 27 females, age 1 to 29 years) from 31 nuclear families were ascertained over a period of more than four years. The incidence of homocystinuria in Qatar was calculated to be > or =1:3000, the highest in the world known so far. All patients in whom data were available were vitamin B6-nonresponsive. Molecular studies were performed in all patients. All 53 patients from tribe M and all three patients from tribe K were homozygous for the mutation c.1006C>T (p.R336C) in the CBS gene, with an additional seven patients resulting from mixed marriages between tribe M and tribe K. A single patient from tribe S was homozygous for mutation c.700G>A (p.D234N) in the CBS gene. Both mutations have been previously reported but involve hypermutable CpG dinculeotides and may be recurrent mutations in the Qatari population. The results of this study illustrate a strong founder effect causing a high prevalence of an autosomal recessive disease in a highly consanguineous Arabian population. Molecular neonatal screening may be suitable for early detection of homocystinuria in this population.

Formulation and release behavior of diclofenac sodium in Compritol 888 matrix beads encapsulated in alginate.

Sustained-release polymer beads containing diclofenac sodium (DNa) dispersed in Compritol 888 and encapsulated in calcium alginate shell were prepared utilizing 2(3) factorial design. The effect of sodium alginate concentration, drug: Compritol 888 weight ratio and CaCl2 concentration on drug content (%), time for 50% and 80% of the drug to be released, and mean dissolution time (MDT) were evaluated with analysis of variance (ANOVA). An increase in the level of all these factors caused retardation in the release, and t50%, t80%, and MDT were increased. The drug release was dependent on the pH of the release media. A formula that gives a release comparable to commercial products was prepared.

Dissolution kinetics of glibenclamide glass.

This work examines the dissolution kinetics of glibenclamide crystalline state, form I, and glibenclamide glassy state, form II. This required determination of the pH-solubility profiles of the two forms, dissolution at different stirring rates, temperatures, and pHs. It was found that form II exhibited higher solubility and dissolution rate at all the studied pHs. However, a significant increase in dissolution and solubility of form II was noted at pH 6. Dissolution studies at different stirring rates confirmed that the dissolution process is diffusion controlled, zero order in nature and adheres to the Noyes and Whitney and Levich equations. Dissolution studies at different temperatures enabled calculation of the heat of dissolution, Ea, of both forms. Ea for form I and form II was found to be almost identical. Storing of form II for one week at different temperatures resulted in the partial transformation of form II to form I as indicated from dissolution studies. The extent of transformation and hence reduction in dissolution rate increased with the increase in storage temperature.

Dissolution behaviour of sustained release formulations of indomethacin with Eudragit RS.

This study investigates the kinetics of the release of indomethacin from Eudragit RS polymer. It involved the study of the dissolution behaviour of indomethacin from different combinations of the drug in a solid dispersion form, granules and physical mixtures. It was evident that Eudragit RS had a retardation effect on the release of indomethacin, this effect was dependent on the amount of Eudragit included. Inclusion of sodium lauryl sulphate (SLS) in the solid dispersion, granules and physical mixtures modified the release and made it comparable to the release from Indocid retard capsules. The kinetics of the release process was found to be best described by the Higuchi square root of time equation and the first order equation indicating that the release process is diffusion controlled and dependent on the initial drug concentration. The retarding effect Eudragit had on the release of indomethacin was attributed to the possible interaction of the ammonium groups of the polymer with the carboxylate anion of indomethacin and to the inclusion of the drug within the inert insoluble polymer matrix.

Bioequivalence study of two capsule formulations of omeprazole.

Bioequivalency of Omeraz, a test-product of Arab Pharmaceutical Manufacturing Company (Jordan) and Losec, a reference product by Astra (Sweden), was evaluated by a randomised crossover study on 22 healthy male volunteers. Serum concentrations of omeprazole were measured by an HPLC assay. There were no statistically significant differences between the two products in terms of serum profile and pharmacokinetic parameters including AUC, Cmax, Tlag, Tmax, MRT, Ke and T1/2. The AUC ratios (Omeraz/Losec) for 24 hours and for infinity were respectively 1.02 with a 90% C.L. of 0.88-1.16 and 1.00 with a 90% C.L. of 0.88-1.14. The two products may be considered as bioequivalent in terms of the extent of absorption as indicated by the AUC ratios. However, the confidence limits of Cmax fall outside the FDA accepted range.

Bioequivalence of two oral dosage forms prepared from different polymorphic modifications of tenoxicam.

The bioavailability of two pharmaceutical dosage forms formulated using different polymorphs of tenoxicam were compared. Serum levels of TNX were analysed using high performance liquid chromatography. The pharmacokinetic parameters were estimated following the oral administration of a single dose (20 mg) of the drug as two different polymorphs to 12 healthy volunteers. The differences between formulations were statistically insignificant.

High performance liquid chromatographic analysis of tetroxoprim and sulphadiazine in serum and urine.

Quantitative determination of tetroxoprim and sulphadiazine in serum and urine was performed using reversed phase high performance liquid chromatography. Protein precipitation using 10% perchloric acid was utilized for purification of serum samples while urine samples were diluted prior to analysis. The mobile phase consisted of triethylammonium acetate buffer (85%), acetonitrile (12%) and methanol (3%), with a final pH of 4.2. The eluent was monitored at 280 nm. Benzoic acid was used as an internal standard. Standardization, validation and application of the method is described.

Microbial contamination and preservation efficacy of cough preparations.

Cough syrups, manufactured by four different Jordanian pharmaceutical companies, were examined for microbial contents and efficacy of preservation from fungal and bacterial contamination. Five per cent of the tested samples were found to be contaminated by Candida albicans and 30% of the products examined did not comply with the pharmacopoeial requirements for optimal preservation from fungal contamination. All the products tested were free from bacteria and were efficiently preserved against accidental bacterial contamination.

Weighted concentration of 137Cs equivalent in foodstuffs in Kuwait from June 1986 to December 1988.

The weighted monthly concentration of 137Cs equivalent (WMC) for various types of foodstuffs imported from June 1986 to December 1988 are discussed. The data presented are based on total concentration of 137Cs equivalent. The concentration was found below the disqualifying level applied in Kuwait. The radioactive contamination was higher in milk and baby milk relative to other types of foodstuffs. The calculation of Kuwait's disqualifying levels are based on the annual dose equivalent of 1 mSv (100 mrem). The measured WMC for most types of foodstuffs represents a small fraction to the annual dose limit recommended for the general public.

High performance liquid chromatographic analysis of caffeine concentrations in plasma and saliva.

A rapid high performance liquid chromatographic (HPLC) method for the analysis of caffeine in plasma and saliva is described. Samples of saliva and plasma were purified using zinc sulphate solution as protein precipitant. The supernatant was injected directly onto the column. The mobile phase consisted of ammonium acetate buffer:acetonitrile:methanol (82:15:3, v/v). Measurements were carried out at 254 nm. Acetanilide was used as the internal standard and analysis was completed in 10 min. No interference from endogenous components or other methylxanthines was observed. The coefficients of variation for within day and between day analysis for both saliva and plasma were less than 7.66%. Samples were collected from 20 volunteers. The correlation coefficient between plasma and saliva caffeine concentrations was found to be 0.98.